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Neu (phospho Tyr1221/Y1222) rabbit pAb
SKU: orb764389
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Neu (phospho Tyr1221/Y1222) |
| Molecular Weight | 180 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-ERBB2 antibody, anti-HER2 antibody, anti-MLN19 antibody, anti-NEU antibody, anti-NGL antibody, anti-Receptor tyrosine-protein kinase erbB-2 antibody, anti-Metastatic lymph node gene 19 protein antibody, anti-MLN 19 antibody, anti-Proto-oncogene Neu antibody, anti-Proto-oncogene c-ErbB-2 antibody, anti-Tyrosine kinase-type cell surface rec antibody
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Neu (phospho Tyr1221/Y1222) rabbit pAb [orb764238]
ELISA, IHC-P, IP, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
50 μl, 100 μl
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Western Blot analysis of various cells using Phospho-Neu (Y1221/Y1222) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HER2 (Phospho-Tyr1221 + Tyr1222) Antibody.
Immunofluorescence analysis of HuvEc cell, using HER2 (Phospho-Tyr1221 + Tyr1222) Antibody. The lane on the right is blocked with the HER2 (Phospho-Tyr1221 + Tyr1222) peptide.
Immunohistochemistry analysis of paraffin-embedded human breast cancer, using HER2 (Phospho-Tyr1221 + Tyr1222) Antibody. The picture on the right is blocked with the HER2 (Phospho-Tyr1221 + Tyr1222) peptide.
Quick Database Links
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Neu (phospho Tyr1221/Y1222) rabbit pAb (orb764389)
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