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Description
Images & Validation
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Item 1 of 2
| Tested Applications | EM, ICC, IP, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as guideline try at 1:2500 for western blot, & 1:500 for IF |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | K20-478-5 |
| Immunogen | Residues 1-459 of human Nesprin-2 |
| Target | Nesprin 2 |
| Purification | Protein G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS + 0.1% sodium azide |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DKFZP434H2235 antibody;DKFZp686E01115 antibody;DKFZp686H1931 antibody;FLJ11014 antibody;FLJ43727 antibody;FLJ45710 antibody;FLJ46790 antibody;KIAA1011 antibody;Nesprin2 antibody;NUA antibody;NUANCE antibody;Nuclear envelope spectrin repeat protein 2 antibody;Nucleus and actin connecting element antibody;Nucleus and actin connecting element protein antibody;Protein NUANCE antibody;Spectrin repeat containing nuclear envelope 2 antibody;Synaptic nuclear envelope protein 2 antibody;Synaptic nuclei expressed gene 2 antibody;SYNE 2 antibody;SYNE2 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
IP
Immunoprecipitation
EM
Electron Microscopy
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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