Nepodin

SKU: orb1220066

Description

Nepodin has antimalarial, and anti-inflammatory activities, it shows significant cyclooxygenase (COX) inhibitory activity. Nepodin has an antidiabetic effect, which is at least partly mediated by stimulation of GLUT4 translocation via AMPK activation by nepodin.To obtain a novel modulator of glucose metabolism, we conducted screening of a small compound library in cultured L6 myotubes. We identified Nepodin that stimulated glucose uptake dose-dependently in differentiated L6 myotubes. The stimulatory effect of Nepodin on glucose uptake was abrogated by a 5'-adenosine monophosphate-activated protein kinase (AMPK) inhibitor. In addition, Nepodin stimulated the phosphorylation of AMPK. Nepodin also stimulated the translocation of GLUT4 to the plasma membrane in L6 myoblasts transfected with a Glut4 cDNA-coding vector and in differentiated L6 myotubes. In in vivo study, Nepodin suppressed the increases in fasting blood glucose levels and improved the glucose intolerance of C57BL/KsJ-db/db mice, a type 2 diabetic animal model. Nepodin rescued the impaired phosphorylation of AMPK in the skeletal muscle of db/db mice.

Key Properties

• CAS Number: 3785-24-8
• MW: 216.23
• Purity: >98% (HPLC)
• Formula: C₁₃H₁₂O₃
• SMILES: CC(=O)C1=C(O)C2=C(C=CC=C2O)C=C1C
• Target: COX
• Solubility: In Vitro: DMSO : 125 mg/mL (578.09 mM)

Bioactivity

• In Vivo:
  Nepodin (Musizin) (orally administration; 2-10 mg/kg; 4 weeks) improves the impaired glucose tolerance of db/db mice, also decreases an increase in plasma insulin concentration of db/db mice in a dose-dependent manner. Animal model: C57BL/KsJ-db/db mice. Dosage: 2 mg/kg; 10 mg/kg. Administration: Orally administration; 2-10 mg/kg; 4 weeks. Result: Had antihyperglycemic potential and improved insulin resistance.
• In Vitro:
  Nepodin (Musizin) exhibits significant IC50 values that are 0.74 and 0.79 μg/ml against P. falciparum chloroquine-sensitive (3D7) and P. falciparum chloroquine-resistant (S20), respectively.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

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Gene Symbol

COX