NEK7 Antibody

• Catalog Number: orb1539163
• Category: Antibodies
• Description: NEK7 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Bovine, Canine, Equine, Gallus, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat
• Immunogen: Peptide with sequence C-NTLANFRIEKK, from the internal region of the protein sequence according to NP_598001.1.
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:64000), WB (0.05 - 0.2 µg/ml)
• Conjugation: Unconjugated
• Target: NEK7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: NEK7, NimA-related protein kinase 7, NIMA-related
• Note: For research use only
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 35kD band observed in lysates of cell line HeLa (calculated MW of 34.6kD according to NP_598001.1). Recommended concentration: 0.05-0.2 ug/ml.
• Expiration Date: 12 months from date of receipt.

NEK7 antibody (0.05 ug/ml) staining of HeLa lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.