Rabbit anti-NEK7 Antibody

SKU: orb1526182

Description

NEK7 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1 and 50
• Target: NEK7
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

serine/threonine-protein kinase Nek7; nimA-related protein kinase 7; NIMA (never in mitosis gene a)-related kinase 7; never in mitosis A-related kinase 7

Detection of human NEK7 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NEK7 antibody orb1526182 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human NEK7 by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NEK7 (Cat. No. orb1526182) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

NEK7

UniProt

Q8TDX7

RefSeq (Protein):NP_598001.1