NEK7 Antibody

SKU: orb125093

Description

Goat polyclonal antibody to NEK7

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.05-0.2 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  WB: Approx 35kDa band observed in lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_598001.1). Recommended concentration: 0.05-0.2µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Thyroid. Recommended concentration: 3.75µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NEK7
• Protein Sequence: NTLANFRIEKK
• Molecular Weight: 34.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NIMA (never in mitosis gene a)-related kinase 7 antibody, anti NIMA-related kinase 7 antibody, anti NEK7 antibody

Western blot analysis of HeLa lysate using NEK7 antibody

Quick Database Links

Gene Symbol

NEK7

NCBI Gene ID

140609