NEK2 Antibody

SKU: orb345429

Description

NEK2 antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000
• Reactivity: Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to NEK2 protein by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein.
• Target: NEK2
• Purity: This product is an affinity-purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. BLAST analysis indicates 100 % homology of the immunizing sequence with NEK2 homologues from human and chimpanzee. Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-NEK2 antibody, Serine threonine protein kinase Nek2 antibody, HSPK 21 antibody, NEK 2 antibody, NEK2A antibody, NIMA, never in mitosis a-related kinase 2 antibody, NimA-like protein kinase 1 antibody, NIMA related kinase 2 antibody, NEK-2, NLK1, NLK-1, NEK2A, HSPK21

Possible model for NEK2 action.

Western blot analysis is shown using Biorbyt's Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.

Western Blot of Rabbit Anti-NEK2 Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker. Lane 2: Jurkat cell lysate (10 µg) spiked with rec. NEK2 protein (50 ng). Lane 3: Jurkat cell lysate (10 µg) spiked with rec. NEK2 protein (20 ng). Lane 4: Jurkat cell lysate (10 µg) spiked with rec. NEK2 protein (20 ng). Lane 5: Jurkat cell lysate (10 µg) (p/n orb348674). Primary Antibody: Anti-NEK2 at 1 µg/ml overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) at 1:70000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644). Expect/Observed MW: ~76kDa rec NEK2.

Quick Database Links

Gene Symbol

NEK2

UniProt

P51955

RefSeq:57791147