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NEFL Antibody
SKU: orb97415
Featured
Description
Images & Validation
−Item 1 of 7
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 1H3A4 |
| Immunogen | Purified recombinant fragment of human NEFL expressed in E. Coli. |
| Target | NEFL |
| Molecular Weight | 62kDa |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
−NFL; NF-L; NF68; CMT1F; CMT2E
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Confocal immunofluorescence analysis of Hela cells using NEFL antibody
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of NEFL antibody
Western blot analysis of Hela (Lane1) and Jurkat (Lane2) cell lysate using NEFL antibody
Western blot analysis of human NEFL (AA: 422-543) recombinant protein using NEFL antibody
Immunohistochemical analysis of formalin fixed and paraffin-embedded lung cancer tissues using NEFL antibody
Flow cytometric analysis of Jurkat cells using NEFL antibody
IHC-P of bladder cancer tissues using NEFL antibody
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
NEFL Antibody (orb97415)
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