NEDD9 Antibody

SKU: orb1258135

Description

NEDD9 Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-174 of human NEDD9 (NP_892011.2).
• Target: NEDD9
• Molecular Weight: Observed: 115kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEDD9, CAS-L, CASL, HEF1, dJ49G10.2, dJ761I2.1, CAS2, CASS2

Western blot analysis of extracts of HeLa cells, using NEDD9 antibody (orb1258135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat kidney using NEDD9 antibody (orb1258135) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using NEDD9 antibody (orb1258135). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

NEDD9

UniProt

Q14511