Necrosulfonamide

SKU: orb1300359

Description

Necrosulfonamide

Research Area

Signal Transduction

Key Properties

• CAS Number: 1360614-48-7
• MW: 461.47
• Purity: 99.49%
• Formula: C₁₈H₁₅N₅O₆S₂
• SMILES: COc1nccnc1NS(=O)(=O)c1ccc(NC(=O)\C=C\c2ccc(s2)[N+]([O-])=O)cc1
• Target: PI4K,MLK,PI3K
• Solubility: DMSO:45 mg/mL (97.51 mM)

Bioactivity

• Target IC50:
  PI3Kγ:5.9 ( IC50 |PI4Kβ:5.9 ( IC50 |PI4KA:8.0 ( IC50 |PI3Kβ:5.9 ( IC50 |PI3Kα:5.8 ( IC50 |PI4Kα:8.0 ( IC50 |PI3Kδ:6.4 ( IC50 |PI4KB:5.9 ( IC50
• In Vitro:
  METHODS: FLAG-tagged RIP3-HT-29 cells were treated with Necrosulfonamide for 6 h, an the interaction between RIP1 and RIP3 was examined using a FLAG-tagged RIP3 pull-down assay. Results: Necrosulfonamide, an inhibitor of necrosis downstream of RIP3, did not bloc the necrosis-induced interaction between RIP1 and RIP3.
• Cell Research:
  Necrosis inhibitors induce diverse effects on MLKL phosphorylation. HT-29 cells are treated with T/S/Z with or without necrosis inhibitors for 12 hr or 8 hr the number of dead cells is determined by measuring released protease activity in culture medium the whole-cell extracts are prepared and analyzed by western blotting the final concentration of 10 μM necrostatin-1 or 1 μM necrosulfonamide are used to block necrosis. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

complex, (E)-Necrosulfonamide, Mixed Lineage Kinase, necrosis(MLKL), necrosome, necrosis, Necrosulfonamide, MLKs, inhibit, formation, Inhibitor, substrate, RIP3

Compound Identifiers

PubChem CID

1566236