NDUFS6 Antibody

• Catalog Number: orb1248921
• Category: Antibodies
• Description: NDUFS6 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-RIRFVGRQKEVNEN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 15kDa band observed in Human Heart lysates and 14kDa in Mouse and Rat Heart lysates (calculated MW of 13.7kDa according to NP_004544.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NDUFS6
• UniProt ID: O75380
• NCBI: NP_004544.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NDUFS6, NADH dehydrogenase (ubiquinone) Fe-S prote
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 15kDa band observed in Human Heart lysates and 14kDa in Mouse and Rat Heart lysates (calculated MW of 13.7kDa according to NP_004544.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

orb1248921 (0.01 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248921 (0.1 ug/ml) staining of Mouse (A) and Rat (B) Heart lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248921 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.