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Item 1 of 5
Item 1 of 5
NDUFC2 Antibody (N-term)
Catalog Number: orb1788359
| Catalog Number | orb1788359 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, IF, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | 5-39 aa |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 14188 Da |
| Target | This NDUFC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-39 amino acids from the N-terminal region of human NDUFC2. |
| UniProt ID | O95298 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | NADH dehydrogenase [ubiquinone] 1 subunit C2, Comp Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded R. kidney section using NDUFC2 Antibody (N-term). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. kidney section using NDUFC2 Antibody (N-term). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells using NDUFC2 Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Fluorescent image of HepG2 cells stained with NDUFC2 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of lysates from Hela cell line, human kidney tissue, HepG2, U-87 MG, U-251 MG cell line (from left to right), using NDUFC2 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
