NDUFC2 Antibody (N-term)

SKU: orb1788359

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This NDUFC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-39 amino acids from the N-terminal region of human NDUFC2. Antigen Region: 5-39 aa.
• Target: NDUFC2 (HGNC:7706)
• Molecular Weight: 14188 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NADH dehydrogenase [ubiquinone] 1 subunit C2, Complex I-B145b, CI-B145b, Human lung cancer oncogene 1 protein, HLC-1, NADH-ubiquinone oxidoreductase subunit B145b, NDUFC2

Immunohistochemical analysis of paraffin-embedded R. kidney section using NDUFC2 Antibody (N-term). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. kidney section using NDUFC2 Antibody (N-term). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of HepG2 cells using NDUFC2 Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Fluorescent image of HepG2 cells stained with NDUFC2 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of lysates from Hela cell line, human kidney tissue, HepG2, U-87 MG, U-251 MG cell line (from left to right), using NDUFC2 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Quick Database Links

Gene Symbol

NDUFC2 (HGNC:7706)

UniProt

O95298