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Item 1 of 4
NDUFB4 Antibody (N-term)
Catalog Number: orb1788454
| Catalog Number | orb1788454 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | 3-36 aa |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:25, WB: 1:1000, IHC-P: 1:25, FC: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 15209 Da |
| Target | This NDUFB4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3-36 amino acids from the N-terminal region of human NDUFB4. |
| UniProt ID | O95168 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | NADH dehydrogenase [ubiquinone] 1 beta subcomplex Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded H. skin section using NDUFB4 Antibody (N-term). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells using NDUFB4 Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from human heart tissue, HepG2, PC-3, Ramos cell line (from left to right), using NDUFB4 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
Fluorescent image of Hela cells stained with NDUFB4 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
