NDUFB10 Antibody

• Catalog Number: orb1257782
• Category: Antibodies
• Description: NDUFB10 Antibody
• Target: NDUFB10
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human NDUFB10 (NP_004539.1).
• UniProt ID: O96000
• MW: Observed: 21kDa
• Tested applications: IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IP: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NADH dehydrogenase [ubiquinone] 1 beta subcomplex
• Research Area: Cancer Biology, Neuroscience, Signal Transduction
• Note: For research use only

Western blot analysis of extracts of various cell lines, using NDUFB10 antibody (orb1257782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded mouse stomach using NDUFB10 antibody (orb1257782) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using NDUFB10 antibody (orb1257782) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using NDUFB10 antibody (orb1257782) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug NDUFB10 antibody (orb1257782). Western blot was performed from the immunoprecipitate using NDUFB10 antibody (orb1257782) at a dilution of 1:1000.