NCS1/Neuronal Calcium Sensor Antibody

• Catalog Number: orb1536303
• Category: Antibodies
• Description: NCS1/Neuronal Calcium Sensor Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Frequenin (recombinant from Mouse with extensive post-translational modifications)
• Concentration: 1 mg/ml
• Dilution range: ELISA (1:10000 - 1:50000), IF (1:100 - 1:2500), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000)
• Conjugation: Unconjugated
• Target: NCS1 / Neuronal Calcium Sensor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Alternative names: NCS1, Frequenin (Drosophila) homolog, FREQ, FLUP,
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B1457 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1457 was determined to be 5 ug/ml.
• Expiration Date: 12 months from date of receipt.