NCOA7 Antibody (N-term)

SKU: orb1927865

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50, FC - 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This NCOA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the N-terminal region of human NCOA7. Antigen Region: 76-103 aa.
• Target: NCOA7
• Molecular Weight: 106162 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nuclear receptor coactivator 7, 140 kDa estrogen receptor-associated protein, Estrogen nuclear receptor coactivator 1, NCOA7, ERAP140, ESNA1

NCOA7 Antibody (N-term) western blot analysis in Hela, MDA-MB-453 cell line and mouse kidney tissue lysates (35 ug/lane).This demonstrates the NCOA7 antibody detected the NCOA7 protein (arrow).

NCOA7 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-NCOA7 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 106 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

NCOA7

UniProt

Q8NI08

RefSeq (Protein):NP_001186550.1, NP_001116314.1, NP_861447.3, NP_001186549.1, NP_001186548.1, NP_001186551.1