NCL Antibody (Center E443)

• Catalog Number: orb29653
• Category: Antibodies
• Description: Rabbit polyclonal antibody to OTX2 (C-term) participates in the development of the brain and belongs to the paired homeobox family, and the Bicoid subfamily. Genetic variations in OTX2 are the cause of microphthalmia syndromic type 5.
• Target: NCL
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: Synthetic Peptide
• UniProt ID: P19338
• MW: 76614
• Tested applications: IF, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, IF/ICC: 1:10-50
• Antibody Type: Primary Antibody
• Clone Number: RB17464
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-RB17464 antibody
• Research Area: Epigenetics
• Note: For research use only
• NCBI: NP_005372.2

Fluorescent confocal image of Hela cell stained with NCL Antibody (Center E443). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly.

NCL Antibody (Center E443) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NCL Antibody (Center E443) for immunohistochemistry. Clinical relevance has not been evaluated.

NCL Antibody (Center E443) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the NCL antibody detected the NCL protein (arrow).

Western blot analysis of NCL (arrow) using rabbit polyclonal NCL Antibody (Center E443). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.