NCF4 Antibody

• Catalog Number: orb1247225
• Category: Antibodies
• Description: NCF4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KDFPEEDDPTN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.0kDa according to NP_000622.2 and 38.8kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration, 2-4ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NCF4
• UniProt ID: Q15080
• NCBI: NP_038202.1, NP_000622.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NCF4, P40PHOX, neutrophil cytosolic factor 4, 40kD
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.0kDa according to NP_000622.2 and 38.8kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration, 2-4ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247225 (0.5 ug/ml) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing P40PHOX and probed with orb1247225 (mock transfection in first lane).

orb1247225 (2.5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.