NCBP2 Antibody

SKU: orb1256482

Description

NCBP2 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human NCBP2 (NP_031388.2).
• Target: NCBP2
• Molecular Weight: Observed: 14kDa
• Purification: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

CBC2, CBP2NIP1, PIG55, nuclear cap-binding protein subunit 2, 20 kDa nuclear cap-binding protein, NCBP 20 kDa subunit, NCBP interacting protein 1, cell proliferation-inducing gene 55 protein, nuclear cap binding protein subunit 2, 20kD, nuclear cap binding protein subunit 2, 20kDa

Western blot analysis of extracts of various cell lines, using NCBP2 antibody (orb1256482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human colon using NCBP2 antibody (orb1256482) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using NCBP2 antibody (orb1256482) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of MCF-7 cells using NCBP2 antibody (orb1256482). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

NCBP2

UniProt

P52298