NaVbeta3 Antibody (FITC)

SKU: orb376981

Description

Mouse monoclonal antibody against NaVbeta3 conjugated to FITC.

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:100 dilution of SMC-490 was sufficient for detection of NavBeta3 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone No.: N396/29 (Formerly sold as S396-29)
• Immunogen: Fusion protein amino acids 1-215 (full-length) of rat NavBeta3
• Target: NaVbeta3
• Molecular Weight: 40kDa
• Purification: Protein G Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NaVbeta3, SCN3B, Scn3b, Sodium channel subunit beta-3, KIAA1158

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta3 Monoclonal Antibody, Clone N396/29. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav beta3 Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav beta3 Antibody (D) Composite.

Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone N396/29. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 μg. Primary Antibody: Mouse Anti-Nav Beta 3 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.

Quick Database Links

Gene Symbol

NaVbeta3

UniProt

Q9JK00

NCBI Gene ID

245956

RefSeq (Protein):NP_620797.1