Nav beta3 Antibody: FITC

• Catalog Number: orb376981
• Category: Antibodies
• Description: Mouse monoclonal antibody against NaVbeta3 conjugated to FITC.
• Target: NaVbeta3
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2B
• Conjugation: FITC
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
• Purification: Protein G Purified
• Immunogen: Fusion protein amino acids 1-215 (full-length) of rat NavBeta3
• UniProt ID: Q9JK00
• MW: 40kDa
• Tested applications: ICC, IF, IHC
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Application notes: A 1:100 dilution of SMC-490 was sufficient for detection of NavBeta3 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: N396/29 (Formerly sold as S396-29)
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Sodium channel subunit beta-3 antibody, Scn3b anti
• Research Area: Neuroscience, Pharmacology & Drug Discovery
• Note: For research use only
• Entrez: 245956
• NCBI: NP_620797.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta3 Monoclonal Antibody, Clone N396/29. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav beta3 Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav beta3 Antibody (D) Composite.

Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone N396/29. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 μg. Primary Antibody: Mouse Anti-Nav Beta 3 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.