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Item 1 of 2
Nav beta3 Antibody: Biotin
Catalog Number: orb376980
| Catalog Number | orb376980 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against NaVbeta3 conjugated to Biotin. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | N396/29 (Formerly sold as S396-29) |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG2B |
| Immunogen | Fusion protein amino acids 1-215 (full-length) of rat NavBeta3 |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000), ICC/IF (1:100) |
| Conjugation | Biotin |
| MW | 40kDa |
| Target | NaVbeta3 |
| Entrez | 245956 |
| UniProt ID | Q9JK00 |
| NCBI | NP_620797.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
| Alternative names | Sodium channel subunit beta-3 antibody, Scn3b anti Read more... |
| Note | For research use only |
| Application notes | A 1:100 dilution of SMC-490 was sufficient for detection of NavBeta3 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta3 Monoclonal Antibody, Clone N396/29. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav beta3 Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav beta3 Antibody (D) Composite.
Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone N396/29. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 μg. Primary Antibody: Mouse Anti-Nav Beta 3 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.
