NASP Antibody (N-term)

• Catalog Number: orb1930769
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB15561
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 85238 Da
• Target: This NASP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the N-terminal region of human NASP.
• UniProt ID: P49321
• NCBI: NP_002473.2, NP_689511.2, NP_001182122.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nuclear autoantigenic sperm protein, NASP, NASP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

NASP Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of NASP (arrow) using rabbit polyclonal NASP Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NASP gene (Lane 2).

All lanes: Anti-NASP Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: human testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 85 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.