Nanos Homologue 1 (NANOS1) Antibody (C-term)

• Catalog Number: orb1436904
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Nanos Homologue 1 (NANOS1).
• Target: NANOS1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Immunogen: Synthetic Peptide
• UniProt ID: Q8WY41
• MW: 30230
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB13839
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nanos homolog 1, NOS-1, EC_Rep1a, NANOS1, NOS1
• Research Area: Signal Transduction, Stem Cell & Developmental Bio
• Note: For research use only
• NCBI: NP_955631.1

Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa Cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Western blot analysis of anti-NANOS1 Antibody (C-term) in T47D cell line lysates (35 ug/lane). NANOS1 (arrow) was detected using the purified Pab.