Nanos Homologue 1 (NANOS1) Antibody (C-term)

SKU: orb1436904

Description

Rabbit polyclonal antibody to Nanos Homologue 1 (NANOS1).

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB13839
• Immunogen: Synthetic Peptide
• Target: NANOS1
• Molecular Weight: 30230
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Nanos homolog 1, NOS-1, EC_Rep1a, NANOS1, NOS1

Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa Cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Western blot analysis of anti-NANOS1 Antibody (C-term) in T47D cell line lysates (35 ug/lane). NANOS1 (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

NANOS1

UniProt

Q8WY41

RefSeq (Protein):NP_955631.1