NANOG Antibody (S285)

SKU: orb1935405

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD30144
• Immunogen: This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-292 amino acids from human NANOG. Antigen Region: 267-292 aa.
• Target: NANOG
• Molecular Weight: 34620 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

All lanes: Anti-NANOG Antibody (S285) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: human testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of SY5Y cells stained with NANOG (S285) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with NANOG (S285) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.

Quick Database Links

Gene Symbol

NANOG

UniProt

Q9H9S0

RefSeq (Protein):NP_079141.2