NANOG Antibody

SKU: orb1272787

Description

NANOG Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Indirect:To detect hNanog by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog. Sandwich:To detect hNanog by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required.Western Blot:To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNanog. Human Nanog specific antibody was purified by affinity chromatography employing immobilized hNanog matrix.
• Target: NANOG
• Purification: Nanog antibody was purified by affinity chromatography employing immobilized Nanog matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

To detect hNanog by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Nanog (orb1272791) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog.

To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human prostate malignant adenocarcinoma. The recommended concentration is 0.5 mg/ml with an overnight incubation at 4 °C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human prostate malignant adenocarcinoma. The recommended concentration is 0.5 mg/ml with an overnight incubation at 4 °C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

NANOG

UniProt

Q9H9S0

RefSeq:Q9H9S0