NANOG Antibody

• Catalog Number: orb1247985
• Category: Antibodies
• Description: NANOG Antibody
• Clonality: Polyclonal
• Tested applications: EIA, ELISA, IF, IHC, WB
• Predicted Reactivity: Canine, Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QNQRMKSKRWQKNN
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NANOG
• UniProt ID: Q9H9S0
• NCBI: NP_079141.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NANOG, Nanog homeobox, HGNC:20857 , homeobox trans
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1;21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22;7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012;21(5):815-25. PMID: 21944493.Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). This antibody was successfully used in IF on Human: Estibaliz Arellano-Viera et al. (2019) https://doi.org/10.1016/j.scr.2019.101410. This anyibody was successfully used in ICC on Human: Stem Cell Research (2019), Enzyme immunoassay: This antibody is reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB07693. This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
• Expiration Date: 12 months from date of receipt.

orb1247985 (0.03 ug/ml) staining of Human Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247985 (5 ug/ml) staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes.