NANOG Antibody

SKU: orb1239398

Description

NANOG Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Gallus
• Application Notes:
  WB: 1-2 μg/mL; ICC/IF: 20 μg/mL; IHC: 1-5 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG.The immunogen is located within amino acids 130 - 180 of NANOG.
• Target: NANOG
• Molecular Weight: Predicted: 35 kD Observed: 35 kD
• Purification: NANOG Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: NANOG Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NANOG Antibody: Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

Immunoflouorescence Validation of NANOG in HeLa Cells. Immunofluorescent analysis of PFA-fixed HeLa cells labeling NANOG with orb1239398 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

WB Validation in HeLa Cells. Loading: 15 µg of lysate. Antibodies: NANOG orb1239398, 2 µg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse and Rat Testis. Loading: 15 µg of lysates per lane. Antibodies: NANOG orb1239398, 2 µg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of NANOG in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-NANOG antibody (orb1239398) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of NANOG in Mouse Testis. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NANOG antibody (orb1239398) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of NANOG in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NANOG antibody (orb1239398) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Quick Database Links

Gene Symbol

NANOG

UniProt

Q9H9S0

RefSeq:EAW88651