NANOG Antibody (Biotin)

SKU: orb1272791

Description

NANOG Antibody (Biotin)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Sandwich:To detect hNanog by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. Western Blot:To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNanog. Human Nanog specific antibody was purified by affinity chromatography and then biotinylated.
• Target: NANOG
• Purification: Nanog antibody was purified by affinity chromatography and then biotinylated.
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

To detect hNanog by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Nanog (orb1272787) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hNanog.

To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

NANOG

UniProt

Q9H9S0

RefSeq:Q9H9S0