NALP3/NLRP3 Antibody

• Catalog Number: orb1538301
• Category: Antibodies
• Description: NALP3/NLRP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: A 16 AA synthetic peptide near the N-Terminus of human NALP3. The immunogen is located within aa 120-170 of NALP3.
• Concentration: 1 mg/ml
• Dilution range: ELISA, ICC (2 µg/ml), IF (20 µg/ml), IHC, IHC-P (2.5 - 5 µg/ml), WB (1 µg/ml)
• Conjugation: Unconjugated
• Target: NALP3 / NLRP3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Alternative names: NLRP3, AGTAVPRL, AII, C1orf7, Caterpiller protein
• Note: For research use only
• Application notes: Further information: NALP3 antibody can be used for detection of NALP3 by Western blot at 1 ug/mL. Antibody can also be used for Immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 101, 114 kDa Observed: 110 kDa
• Expiration Date: 12 months from date of receipt.

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot of NLRP3 in K562 cell lysate with NLRP3 antibody at 1 ug/ml (A) in the absence and (B) in the presence of blocking peptide.