NaK ATPase alpha 1/ATP1A1 Antibody

• Catalog Number: orb1294398
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Sodium Potassium ATPase
• Target: NaK ATPase alpha 1 / ATP1A1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the N-terminus region.
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:100-1:200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Anti-Na/K ATPase antibody at 1/700 dilution, Lysates/proteins at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294398 overnight at 4°C.

Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294398 anti-Na/K ATPase antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded human cancer tissue labeling Na/K ATPase with orb1294398 at 1/100.