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N4BP1 Antibody

Catalog Number: orb1239410

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1239410
CategoryAntibodies
DescriptionN4BP1 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, ICC, IF, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenN4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1.The immunogen is located within the first 50 amino acids of N4BP1.
Antibody TypePrimary Antibody
Concentration1 mg/mL
Form/AppearanceLiquid
ConjugationUnconjugated
TargetN4BP1
UniProt IDO75113
NCBINP_694574
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesN4BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Alternative namesN4BP1 Antibody: KIAA0615, NEDD4-binding protein 1,
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NoteFor research use only
Application notesN4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Expiration Date12 months from date of receipt.
N4BP1 Antibody

WB Validation in Human Cell LinesLoading: 15 µg of lysates Antibodies: N4BP1, orb1239410 1 µg/ml, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Exposure: 30 sec.

N4BP1 Antibody

Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 µg/mL.

N4BP1 Antibody

Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 µg/mL.

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