N4BP1 Antibody

• Catalog Number: orb1239410
• Category: Antibodies
• Description: N4BP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1.The immunogen is located within the first 50 amino acids of N4BP1.
• Concentration: 1 mg/mL
• Dilution range: N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: N4BP1
• UniProt ID: O75113
• NCBI: NP_694574
• Storage: N4BP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: N4BP1 Antibody: KIAA0615, NEDD4-binding protein 1,
• Note: For research use only
• Application notes: N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

WB Validation in Human Cell LinesLoading: 15 µg of lysates Antibodies: N4BP1, orb1239410 1 µg/ml, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Exposure: 30 sec.

Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 µg/mL.

Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 µg/mL.