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Catalog Number | orb1239410 |
---|---|
Category | Antibodies |
Description | N4BP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1.The immunogen is located within the first 50 amino acids of N4BP1. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | N4BP1 |
UniProt ID | O75113 |
NCBI | NP_694574 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | N4BP1 Antibody: KIAA0615, NEDD4-binding protein 1, Read more... |
Note | For research use only |
Application notes | N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
WB Validation in Human Cell LinesLoading: 15 µg of lysates Antibodies: N4BP1, orb1239410 1 µg/ml, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Exposure: 30 sec.
Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 µg/mL.
Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 µg/mL.
ELISA, FC, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |