N-terminal Arginylation Antibody: HRP

• Catalog Number: orb396275
• Category: Antibodies
• Description: Mouse monoclonal antibody against Arginylation conjugated to HRP.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4A9
• Tested applications: ELISA, IHC, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: Synthetic N-terminal arginylated peptide conjugated to KLH
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); FCM (1:50)
• Conjugation: HRP
• Target: N-terminal Arginylation
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: N-terminal Arginine antibody, N-terminal Arginylat
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-263 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band (s): 250kDa, 150kDa, 75kDa REHKH-BSA.

Flow Cytometry analysis using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Heat stressed cells were subject to heat shock at 42°C for 2 hours.