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CDH2 Antibody
SKU: orb1274458
Featured
Description
Research Area
Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 750-850 of human N Cadherin (NP_001783.2). |
| Target | CDH2 |
| Molecular Weight | Observed: 135kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CDH2, CDw325, CDHN, Cadherin-2, CD325, CD325 antigen, N Cadherin, N-cadherin, NCAD, Neural cadherin, N-cadherin 1, Neural-cadherin
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Western blot analysis of extracts of various cell lines, using N Cadherin antibody (orb1274458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts from normal (control) and N Cadherin knockout (KO) HeLa cells, using N Cadherin antibody (orb1274458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug N Cadherin antibody (orb1274458). Western blot was performed from the immunoprecipitate using N Cadherin antibody (orb1274458) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
CDH2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
CDH2 Antibody (orb1274458)
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