CD325 Antibody / CDH2 / N-Cadherin

SKU: orb2636914

Description

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Optimal dilution of the CD325 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone No.: MNCD2
• Immunogen: A portion of amino acids 308-597 was used as the immunogen for the CD325 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human heart tissue with CD325 antibody (clone MNCD2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human heart tissue with CD325 antibody (clone MNCD2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P19022