TLCK

SKU: orb1307996

Description

N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) is a cell-permeable, irreversible inhibitor of trypsin-like serine proteases. It is widely used in vitro to study protease function and has been applied in research models to investigate the role of such enzymes in inflammation and immune responses, including IFN-γ-mediated pathways.

Research Area

Cell Biology

Key Properties

• CAS Number: 4272-74-6
• MW: 369.3
• Purity: >98%
• Formula: C₁₄H₂₁ClN₂O₃S • HCl
• SMILES: O=S(C1=CC=C(C)C=C1)(N[C@H](C(CCl)=O)CCCCN)=O.[H]Cl
• Target: Protease
• Solubility: Soluble in Water (up to 50 mg/ml).

Bioactivity

• In Vitro:
  A lower concentration of anti-Fas (10 ng/mL) is used to examine the interaction among the three effectors simultaneously, that is, anti-Fas, TLCK, and IFN-γ. N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride (50 μM) exhibits a small decrease in cell viability. Beyond 50 μM, a dose-dependent decrease in cell viability is observed. IFN-γ slightly reduces cell viability on its own. The addition of anti-Fas (10 ng/mL) results in a slight decrease in cell survival, which is enhanced more than additively in the presence of TLCK, most prominently between 50 and 100 μM. Upon addition of both anti-Fas and IFN-γ, a decrease (≈46%) in cell viability is observed. Moreover, the decrease in cell survival is further enhanced by the addition of higher concentrations of TLCK, 25 μM, and more.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

N-[(3S)-7-Amino-1-chloro-2-oxoheptan-3-yl]-4-methylbenzenesulfonamide, hydrochloride

Compound Identifiers

PubChem CID

73093