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Catalog Number | orb1929427 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB07896 |
Tested applications | WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500, WB: 1:500, WB: 1:500, WB: 1:2000, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 54521 Da |
Target | This MYT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T495 of human MYT1. |
UniProt ID | Q99640 |
NCBI | NP_001245380.1, NP_004194.3, NP_001245379.1, NP_872629.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Membrane-associated tyrosine- and threonine-specif Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of anti-MYT1 Antibody (C-term) in HL60 cell line lysates (35 ug/lane). MYT1 (arrow) was detected using the purified Pab (1:60 dilution).
All lanes: Anti-MYT1 Antibody (C-term) at 1:500 dilution. Lane 1 Mouse testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 55kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-MYT1 Antibody (C-term) at 1:500 dilution. Lane1: Mouse testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 55kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-MYT1 Antibody (C-term) at 1:2000 dilution. Lane 1: SK-BR-3whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 55kDa. Blocking/Dilution buffer: 5% NFDM/TBST.