MyoD1 Antibody (Center)

SKU: orb1937301

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:25, WB - 1:4000
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB18817
• Immunogen: This MyoD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human MyoD1. Antigen Region: 211-240 aa.
• Target: MYOD1
• Molecular Weight: 34501 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3, MYOD1, BHLHC1, MYF3, MYOD

All lanes: Anti-MyoD1 Antibody (Center) at 1:4000 dilution. Lane 1: Human skeletal muscle tissue lysate. Lane 2: Human placenta tissue lysate. Lane 3: RD whole cell lysate. Lane 4: HL-60 whole cell lysate. Lane 5: Hela whole cell lysate. Lane 6: HT-1080 whole cell lysate. Lane 7: U-2OS whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling MYOD1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus staining on Hela cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (red). The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

MYOD1

UniProt

P15172

RefSeq (Protein):NP_002469.2