MYO6 Antibody (C-term R1181)

• Catalog Number: orb1938104
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This MYO6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1166-1195 amino acids from the C-terminal region of human MYO6.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9UM54
• MW: 149691 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:2000-1:8000, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB19127
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Unconventional myosin-VI, Unconventional myosin-6,
• Note: For research use only
• NCBI: NP_004990.3

All lanes: Anti-MYO6 Antibody (C-term R1181) at 1:2000-1:8000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HCT116 whole cell lysate. Lane 3: HT-29 whole cell lysate. Lane 4: LNCap whole cell lysate. Lane 5: MCF-7 whole cell lysate. Lane 6: ZR-75-1 whole cell lysate. Lane 7: Human brain lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 150 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of MYO6 Antibody (C-term R1181) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

MYO6 Antibody (C-term R1181) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MYO6 Antibody (C-term R1181) for immunohistochemistry. Clinical relevance has not been evaluated.