MYO5A Antibody

• Catalog Number: orb1248882
• Category: Antibodies
• Description: MYO5A Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-ETKQLELDLN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3ug/ml.Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2031-6; PMID: 20133847). Recommended concentration: 1-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MYO5A
• UniProt ID: Q9Y4I1
• NCBI: NP_001135967.1, NP_000250.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: MYO5A, myosin VA (heavy chain 12, myoxin), GS1, MY
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3ug/ml.Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2031-6; PMID: 20133847). Recommended concentration: 1-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248882 (1 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248882 (1 ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence.