MYO1B Antibody

SKU: orb1248837

Description

MYO1B Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 120kDa band observed in lysates of cell line NIH3T3 (calculated MW of 125kDa according to Human NP_036355.2 and 128kDa according to Mouse NP_034993.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in the neuronal bodies. Recommended concentration, 5-10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RTVEAKQEKVSTT
• Target: MYO1B
• Molecular Weight: Approx 120 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

MYO1B, myosin IB, myr1, MMI-alpha, MMIa, MYH-1c, MYO1B variant protein, OTTHUMP00000165861, myosin I alpha, myosin-I alpha, myosin-Ib

orb1248837 (1 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248837 (5 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

MYO1B

UniProt

O43795

RefSeq (Protein):NP_001123630.1, NP_036355.2