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Description
Research Area
Musculoskeletal & Connective Tissue Research
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD55953 |
| Immunogen | This MYL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MYL3. Antigen Region: 1-30 aa. |
| Target | MYL3 (HGNC:7584) |
| Molecular Weight | 21932 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Myosin light chain 3, Cardiac myosin light chain 1, CMLC1, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MLC1SB, Ventricular/slow twitch myosin alkali light chain, MYL3
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Western blot analysis of lysates from human skeletal muscle and heart tissue lysates (from left to right), using MYL3 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
MYL3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MYL3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000249.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
MYL3 Antibody (N-term) (orb1935766)
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