MYL2 rabbit pAb Antibody

SKU: orb771737

Description

Rabbit polyclonal antibody to MYL2

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthesized peptide derived from human MYL2. at AA range: 91-140
• Molecular Weight: 18
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-Myosin regulatory light chain 2 antibody, anti-ventricular/cardiac muscle isoform (MLC-2) (MLC-2v) antibody

Western Blot analysis of 1, mouse-heart 2, Hela cells using primary antibody diluted at 1:500 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour) .

Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).