MYH6 Recombinant Rabbit Monoclonal Antibody

SKU: orb1610346

Description

MYH6 Recombinant Rabbit Monoclonal Antibody

Research Area

Autoimmune, Cytokinesis, Immunology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 8C1
• Immunogen: A synthesized peptide derived from human Myosin heavy chain 6 (1100-1400/1935aa)
• Target: MYH6
• Molecular Weight: 224 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1*TBS (pH7.4), 0.05% rAlbumin, 40% Glycerol. Preservative: 0.02% Proclin300.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

MYH6_HUMAN; Myosin-6; MYHCA; Myosin heavy chain 6; Myosin heavy chain, cardiac muscle alpha isoform(MyHC-alpha); ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC; Myosin heavy chain;

25 ug total protein per lane of various lysates probed with MYH6 monoclonal antibody, unconjugated (orb1610346) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MYH6 Monoclonal Antibody, Unconjugated (orb1610346) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MYH6 Monoclonal Antibody, Unconjugated (orb1610346) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MYH6 Monoclonal Antibody, Unconjugated (orb1610346) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MYH6 Monoclonal Antibody, Unconjugated (orb1610346) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

MYH6

UniProt

P13533