MYH2 Antibody

• Catalog Number: orb1259583
• Category: Antibodies
• Description: MYH2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human MYH2 (NP_001093582.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 223kDa
• Target: MYH2
• UniProt ID: Q9UKX2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: IBM3A, MYHSA2, MYHas8, MYPOP, MyHC-2A, MyHC-IIa, M
• Note: For research use only
• Application notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using MYH2 antibody (orb1259583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Immunofluorescence analysis of mouse skeletal muscle using MYH2 Polyclonal Antibody (orb1259583) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.