Myc Tag Antibody

SKU: orb1927588

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:2000

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 9E10
• Immunogen: KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tag from c-Myc protein

Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody. Diluted at 1:2000. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysate at 20 ug.

Anti-Myc Tag Antibody at 1:2000 dilution + 12 tag recombinant protein lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.