TRIM54 Antibody

SKU: orb1247238

Description

TRIM54 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Experiments gave bands at approx 45kDa and 38kDa in Human Skeletal Muscle lysate after 1µg/mL antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3µg/mL.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-GSAGPEEERPDGP
• Target: TRIM54
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TRIM54, MURF-3, tripartite motif-containing 54, RNF30, MURF, ring finger protein 30, muscle-specific RING-finger protein 3, muscle-specific RING-finger protein homolog, OTTHUMP00000122598

Quick Database Links

Gene Symbol

TRIM54

UniProt

Q9BYV2

RefSeq (Protein):NP_912730.2, NP_115935.3