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Catalog Number | orb534021 |
---|---|
Category | Antibodies |
Description | Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | CTKNM-1R |
Tested applications | IF, IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | Human epithelial cell keratin was used as the immunogen for the recombinant Multi Cytokeratin antibody. |
Dilution range | Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant Multi Cytokeratin antibody is available for research use only. |
Storage | Store the recombinant Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant Multi Cytokeratin antibody should be determined by the researcher.1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human skin with recombinant Multi Cytokeratin antibody (clone CTKNM-1R). HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
FACS, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Bovine, Frog, Goat, Guinea pig, Human, Marmoset, Mouse, Porcine, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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