MUC4 Antibody / Mucin-4

SKU: orb2642786

Description

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. MUC-4 transcripts have been detected in normal respiratory epithelium and lung. MUC-4 is a very specific (100%) and sensitive (90%) marker of lung adenocarcinomas and is negative for mesotheliomas. Reportedly, MUC-4 expression in invasive ductal carcinoma of the pancreas is an independent factor for poor prognosis.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC4 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: MUC4/3084
• Immunogen: Amino acids 1730-1864 from the human protein were used as the immunogen for this MUC4 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human colon carcinoma with MUC4 antibody (clone MUC4/3084). HIER: requires steaming of sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE human gastric carcinoma with MUC4 antibody (clone MUC4/3084). HIER: requires steaming of sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE human gastric carcinoma with MUC4 antibody (clone MUC4/3084). HIER: requires steaming of sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free MUC4 antibody (clone MUC4/3084) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q99102