MUC3A Antibody

SKU: orb1252484

Description

MUC3A Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the MUC3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: MUC3/1154
• Immunogen: Amino acids (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS) which contains two of the MUC3 tandem repeats was used as the immunogen for the MUC3 antibody.
• Target: MUC3A
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human gastric carcinoma stained with MUC3 antibody (MUC3/1154).

Formalin-fixed, paraffin-embedded human gastric carcinoma stained with MUC3 antibody (MUC3/1154).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with MUC3 antibody (MUC3/1154).

Quick Database Links

Gene Symbol

MUC3A

UniProt

Q02505