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Catalog Number | orb1262922 |
---|---|
Category | Antibodies |
Description | MUC20 Antibody |
Target | MUC20 |
Clonality | Polyclonal |
Isotype | Rabbit Ig |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Immunogen | This MUC20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 654-684 amino acids from the C-terminal region of human MUC20. |
UniProt ID | Q8N307 |
MW | 72 kDa |
Tested applications | FC, IF, IHC-P, WB |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Mucin-20, MUC-20, MUC20, KIAA1359 |
Note | For research use only |
NCBI | Q8N307 |
Western blot analysis in WiDr cell line lysates (35 ug/lane).
MUC20 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of MUC20 Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |